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ssdna probes  (Zymo Research)


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    Zymo Research ssdna probes
    Ssdna Probes, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 4179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssdna+probes/10__1158_slash_2643___3230__bcd___25___0315-386-134-136?v=Zymo+Research
    Average 99 stars, based on 4179 article reviews
    ssdna probes - by Bioz Stars, 2026-08
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    Image Search Results


    SCas12a system enables PAM- and cis- cleavage-free detection of DNA. ( a ) Schematic illustration of the SCas12a assay for detecting RNA and DNA targets. ( b ) Cis-cleavage of target dsDNA plasmids by As, Lb, and Ct Cas12a proteins, with or without a PAM sequence (TTTG). The reactions contained 100 nM Cas12a, 200 nM split crRNA, and 10 nM target dsDNA plasmids containing HPV16 target sequences. ( c ) Fluorescence-based detection of WT Cas12a’s trans-cleavage activity in the presence of target dsDNA substrates containing canonical, suboptimal, or absent PAM sequences, using a complete crRNA targeting the gene fragment of HPV16. The reactions contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( d ) Fluorescence-based detection of SCas12a system’s trans-cleavage activity in the presence of target dsDNA substrates containing canonical, suboptimal, or absent PAM sequences, using a split crRNA targeting the gene fragment of HPV16. The reactions contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( e ) Heatmap representing a comparison of the trans-cleavage activity between the SCas12a and WT Cas12a systems on identical DNA substrates with or without PAM sequences. ( f ) Detection of PAM-free dsDNA substrates by As, Lb, and Ct Cas12a proteins. The reaction mixtures were incubated for 60 min at 37°C for AsCas12a and LbCas12a, and at 55°C for CtCas12a, and contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( g ) Comparison of the maximum fluorescence values among As, Lb, and Ct Cas12a in Fig. . Mean ± s.d. of n = 3 technical replicates for panels (c–g). a.u., arbitrary units.

    Journal: Nucleic Acids Research

    Article Title: Elimination of cis -cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection

    doi: 10.1093/nar/gkag267

    Figure Lengend Snippet: SCas12a system enables PAM- and cis- cleavage-free detection of DNA. ( a ) Schematic illustration of the SCas12a assay for detecting RNA and DNA targets. ( b ) Cis-cleavage of target dsDNA plasmids by As, Lb, and Ct Cas12a proteins, with or without a PAM sequence (TTTG). The reactions contained 100 nM Cas12a, 200 nM split crRNA, and 10 nM target dsDNA plasmids containing HPV16 target sequences. ( c ) Fluorescence-based detection of WT Cas12a’s trans-cleavage activity in the presence of target dsDNA substrates containing canonical, suboptimal, or absent PAM sequences, using a complete crRNA targeting the gene fragment of HPV16. The reactions contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( d ) Fluorescence-based detection of SCas12a system’s trans-cleavage activity in the presence of target dsDNA substrates containing canonical, suboptimal, or absent PAM sequences, using a split crRNA targeting the gene fragment of HPV16. The reactions contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( e ) Heatmap representing a comparison of the trans-cleavage activity between the SCas12a and WT Cas12a systems on identical DNA substrates with or without PAM sequences. ( f ) Detection of PAM-free dsDNA substrates by As, Lb, and Ct Cas12a proteins. The reaction mixtures were incubated for 60 min at 37°C for AsCas12a and LbCas12a, and at 55°C for CtCas12a, and contained 250 nM Cas12a, 500 nM crRNA, 10 nM target dsDNA substrate, and 1000 nM FAM-labeled ssDNA fluorescence probes. ( g ) Comparison of the maximum fluorescence values among As, Lb, and Ct Cas12a in Fig. . Mean ± s.d. of n = 3 technical replicates for panels (c–g). a.u., arbitrary units.

    Article Snippet: FAM-labeled ssDNA probes, DNA and RNA oligonucleotides, and gene fragments were synthesized by Sangon Biotech (Shanghai, China).

    Techniques: Sequencing, Fluorescence, Activity Assay, Labeling, Comparison, Incubation